CD45.1 antibody (violetFluor™ 450)

Catalog No. ABIN2567751

The Mouse Monoclonal anti-CD45.1 antibody has been validated for FACS. It is suitable to detect CD45.1 in samples from Mouse.

Quick Overview for CD45.1 antibody (violetFluor™ 450) (ABIN2567751)

Target: CD45.1 (CD45 (CD45.1 Allogen))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD45.1 antibody is conjugated to violetFluor™ 450

Application: Flow Cytometry (FACS)

Clone: A20

Product Details anti-CD45.1 Antibody (violetFluor™ 450)

Specificity: The A20 monoclonal antibody reacts with the leukocytes of the CD45.1-expressing mouse strains (DA, SJL/J, RIII, and STS/A).

Purification: The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: Phosphate-buffered aqueous solution, ≤0.09 % Sodium azide, may contain carrier protein/stabilizer, ph7.2.

Storage: 4 °C

Storage Comment: The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze.

Target Details for CD45.1

Target: CD45.1 (CD45 (CD45.1 Allogen))

Alternative Name: CD45.1

Background: The A20 monoclonal antibody reacts with the leukocytes of the CD45.1-expressing mouse strains (DA, SJL/J, RIII, and STS/A). It does not cross-react with cells that express CD45.2. The CD45 Molecule is a member of the Protein Tyrosine Phosphatase (PTP) family, because its intracellular region contains two PTP domains. The extracellular region's variability is caused by different levels of glycosylation, and the splicing of the 4, 5, and 6 exons. The isoforms found in the mouse strains depend on the activation state, maturation stage and cell type, and are very important in B and T lymphocytes antigen receptor signal transduction. The A20 antibody inhibits some of the B lymphocytes responses, from CD45.1-expressing mice, to lipopolysaccharides and antigens. BG Violet 450 conjugate is an alternative to the Pacific Blue, eFluor 450, or BD Horizon V450 dyes. It is excited by the violet (405 nm) laser, with a peak emission of 450nm.

Gene ID: 19264

UniProt: P06800